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Seca d133n mutant
D133n Mutant, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d133n+mutant/seca+d133n/pmc04684309-201-7-31
Average 90 stars, based on 1 article reviews
d133n mutant - by Bioz Stars, 2026-09
90/100 stars

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Mutagenesis:

Article Title: Role of a Conserved Glutamate Residue in the Escherichia coli SecA ATPase Mechanism
Article Snippet: .. As expected from the ATPase results, the D133N mutant is unable to catalyze protein translocation ( , lane 2 ), whereas D133A mutant activity is indistinguishable from that of wild type SecA ( , lane 3 ). .. It is possible that with SecA-D133N, as in the case of SecA-D210A and SecA-D210Q, some perturbation other than that of catalytic chemistry results in loss of activity.

Translocation Assay:

Article Title: Role of a Conserved Glutamate Residue in the Escherichia coli SecA ATPase Mechanism
Article Snippet: .. As expected from the ATPase results, the D133N mutant is unable to catalyze protein translocation ( , lane 2 ), whereas D133A mutant activity is indistinguishable from that of wild type SecA ( , lane 3 ). .. It is possible that with SecA-D133N, as in the case of SecA-D210A and SecA-D210Q, some perturbation other than that of catalytic chemistry results in loss of activity.

Activity Assay:

Article Title: Role of a Conserved Glutamate Residue in the Escherichia coli SecA ATPase Mechanism
Article Snippet: .. As expected from the ATPase results, the D133N mutant is unable to catalyze protein translocation ( , lane 2 ), whereas D133A mutant activity is indistinguishable from that of wild type SecA ( , lane 3 ). .. It is possible that with SecA-D133N, as in the case of SecA-D210A and SecA-D210Q, some perturbation other than that of catalytic chemistry results in loss of activity.



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Seca d133n mutant
D133n Mutant, supplied by Seca, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d133n+mutant/seca+d133n/pmc04684309-201-7-31
Average 90 stars, based on 1 article reviews
d133n mutant - by Bioz Stars, 2026-09
90/100 stars
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